Confocal microcopy is and has been a mainstay of light microcopy for decades given the flexibility of a commercially available system. However, the resolution of a confocal microscope is still diffraction limited with a lateral resolution of ~250nm. With the recent advent of superresolution techniques (PALM, STORM, STED, and SIM), new commercially available systems are being used to image beyond the diffraction limit. As a result, the increased spatial resolution novel information regarding cell structure and function can be obtained. Recently the new Zeiss Airyscan detector was introduced as another method of superresolution and represents a good blend of resolution, sensitivity and speed. In this session, Dr.Xufeng Wu will present her recent experiences using the Airyscan to image cytoskeletal dynamics, organelle and membrane trafficking, and three-dimensional tissue architecture.